CLOT ACTIVATOR&ORSIN-High quality Cost-effective Blood Collection Tube

III. Key Supplementary Notes

1. Separation Gel Advantages:

   • Japanese-imported gel with precise density (between serum and blood cells) forms solid barrier post-centrifugation, blocking molecular exchange (>48h serum stability).

   • Temperature-resistant (up to 45°C) and gamma-irradiated for sterilization; resistant to degradation during transport.

2. Limitations for Drug/Hormone Testing:

   • Potential concentration deviations due to diffusion of small-molecule drugs/hormones into gel layer; tube compatibility verification recommended.

3. Operational Protocols:

   • Immediate mixing after blood draw is critical for uniform activator contact;

   • Low-temperature environments (<16°C) require extended clotting time or increased activator concentration.

     Frequently Asked Questions (FAQ)

     Question	Answer & Solution
     1. Fuzzy separation/milky serum after centrifugation?	Cause: Incomplete clotting or incorrect centrifugation parameters. Solution: - Ensure clotting temperature ≥16°C (≥30 min incubation); - Centrifugation ≥3500 rpm × 5 min; - Avoid hemolysis (rough handling/poor draw).
     2. Gel layer floating or sinking?	Cause: High temperature (>45°C) or gel-sample density mismatch. Solution: - Strictly store/transport at 4-25°C;
     3. Prolonged clotting time (>30 min)?	Cause: Low ambient temperature or expired activator. Solution: - Extend incubation to 60 min in cold environments or transfer to 25°C; - Verify expiry date; discard expired tubes.
     4. Underestimated drug concentrations?	⚠️ Critical Alert: Small-molecule drugs (e.g., warfarin, antibiotics) may diffuse into gel! Solution: - Use dedicated gel-free tubes for TDM; - If used, complete testing within 4h and note "potential underestimation".
     5. Suitable for coagulation tests (PT/APTT)?	❌ Prohibited: Activators interfere with clotting factors! Correct Choice: - Use sodium citrate tubes (blue cap) for coagulation tests.
     6. Stability period of separated serum?	Stability: - RT (15-25°C): ≤48h; - Refrigerated (2-8°C): ≤7 days; - Frozen (-20°C): ≤3 months (transfer to cryovials).
     7. Risks of delayed mixing post-draw?	Risk: Incomplete activation → fibrin formation → probe clogging! Protocol: Gently invert tube ≥8 times within 5 seconds after draw.

     Critical Operational Warnings

      

     NEVER FREEZE: Gel permanently degrades upon freezing!

      

     AVOID >30°C: Accelerates activator degradation during storage/transport.

      

     Pre-centrifugation check: Incomplete clotting causes cellular contamination!

      

     Post-centrifugation: Wait 10 minutes before opening to stabilize gel barrier.